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transwell permeable supports 3422 (Corning Life Sciences)

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Structured Review

Corning Life Sciences transwell permeable supports 3422

(A) Relative cell viability via Cell Titer Glo in HTHM, ADC106, and SVR cells treated with indicated dose of enoxacin (ENX) for 72 hours. (B) Relative Caspase 3/7 activity in ADC106, SVR, and HTHM cells treated with DMSO or 100 µM ENX for 48 hours. (C) Population doubling assays in ADC106 and SVR cells treated with DMSO or 100µm ENX. (D) Representative images and (E) quantification of clonogenic colony formation in cells treated with DMSO or 100 µM ENX. (F) Representative image and (G) quantification of migrated cells in <t>transwell</t> migration assays in cells treated with DMSO or 100 µM ENX, data presented as the mean of four biological replicate experiments with standard error of mean (SEM), *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Transwell Permeable Supports 3422, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transwell+permeable+supports+3422/96+well+plates/bio_rxiv__2025__07__15__664801-134-5-9
Average 90 stars, based on 1 article reviews

transwell permeable supports 3422 - by Bioz Stars, 2026-09

90/100 stars

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1) Product Images from "Canonical microRNA loss drives tumor development implicating therapeutic efficacy of enoxacin in angiosarcoma"

Article Title: Canonical microRNA loss drives tumor development implicating therapeutic efficacy of enoxacin in angiosarcoma

Journal: bioRxiv

doi: 10.1101/2025.07.15.664801

Figure Legend Snippet: (A) Relative cell viability via Cell Titer Glo in HTHM, ADC106, and SVR cells treated with indicated dose of enoxacin (ENX) for 72 hours. (B) Relative Caspase 3/7 activity in ADC106, SVR, and HTHM cells treated with DMSO or 100 µM ENX for 48 hours. (C) Population doubling assays in ADC106 and SVR cells treated with DMSO or 100µm ENX. (D) Representative images and (E) quantification of clonogenic colony formation in cells treated with DMSO or 100 µM ENX. (F) Representative image and (G) quantification of migrated cells in transwell migration assays in cells treated with DMSO or 100 µM ENX, data presented as the mean of four biological replicate experiments with standard error of mean (SEM), *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Techniques Used: Activity Assay, Migration

Membrane:

Article Title: HELLS modulates the stemness of intrahepatic cholangiocarcinoma through promoting senescence-associated secretory phenotype
Article Snippet: .. To investigate the invasion ability, 50 μl matrigel (356234, BD Biosciences, New Jersey, USA) diluted at 1:8 with RPMI-1640 medium was added to the microporous membrane of Transwell Permeable Supports (3422, Corning Inc., New York, USA). ..

Article Title: Alisertib impairs the stemness of hepatocellular carcinoma by inhibiting purine synthesis.
Article Snippet: .. In detailed, 50 μl matrigel (356234, BD Biosciences, New Jersey, USA) diluted at 1:8 with DMEM medium was added to the microporous membrane of Transwell Permeable Supports (3422, Corning Inc., New York, USA) in order to investigate the invasion ability. ..

Article Title: Alisertib impairs the stemness of hepatocellular carcinoma by inhibiting purine synthesis
Article Snippet: .. In detail, 50 μl Matrigel (356234; BD Biosciences) diluted at 1:8 with DMEM was added to the microporous membrane of Transwell Permeable Supports (3422; Corning, Inc) in order to investigate the invasion ability. ..

Migration:

Article Title: Design, Synthesis, and Evaluation of Trihalomethyl Ketone Derivatives of Neocarzilin A as Improved Antimetastatic Agents
Article Snippet: The area of each scratch was calculated using ImageJ software and compared to the area of the scratch at t = 0 h. Percent migration was normalized to that of the DMSO control. .. Transwell migration assays were performed with transwell permeable supports (3422, Corning) with 2.5 × 10 4 cells suspended in serum-free DMEM. ..

Article Title: Identifying KDM5B as the synthetic lethal target of KMT2D-mutated osteosarcoma.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

other:

Article Title: Transforming Growth Factor Beta2 Promotes Migration and Inhibits the Proliferation of Gastric Cancer Cells by Regulating the pSmad2/3-NDRG1 Signaling Pathway.
Article Snippet: The study utilized Transwell Permeable Supports (3422, Corning) for migration assays, with each group repeated at least three times.

Transwell Migration Assay:

Image Search Results

Journal: bioRxiv

Article Title: Canonical microRNA loss drives tumor development implicating therapeutic efficacy of enoxacin in angiosarcoma

doi: 10.1101/2025.07.15.664801

Figure Lengend Snippet: (A) Relative cell viability via Cell Titer Glo in HTHM, ADC106, and SVR cells treated with indicated dose of enoxacin (ENX) for 72 hours. (B) Relative Caspase 3/7 activity in ADC106, SVR, and HTHM cells treated with DMSO or 100 µM ENX for 48 hours. (C) Population doubling assays in ADC106 and SVR cells treated with DMSO or 100µm ENX. (D) Representative images and (E) quantification of clonogenic colony formation in cells treated with DMSO or 100 µM ENX. (F) Representative image and (G) quantification of migrated cells in transwell migration assays in cells treated with DMSO or 100 µM ENX, data presented as the mean of four biological replicate experiments with standard error of mean (SEM), *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Article Snippet: Transwell assays were performed with transwell permeable supports (3422, Corning, Glendale, AZ, USA).

Techniques: Activity Assay, Migration

a – d CRC cells were exposed to specific siRNAs or plasmids. RNA level, protein level, cell viability and migration capability were detected by RT-qPCR ( a ), immunoblot ( b ), CCK-8 ( c ) and Transwell ( d ) assays, respectively. e HCT116 cells transduced with lentivirus vectors were subcutaneously inoculated into Balb/c nude mice ( n = 5). Tumors were stripped, weighed and measured three weeks after the implantations. Data were presented as the mean ± SD. ALY ALYREF. ** P < 0.01; *** P < 0.001; two-sided Student’s t test.

Journal: NPJ Precision Oncology

Article Title: ALYREF recruits ELAVL1 to promote colorectal tumorigenesis via facilitating RNA m5C recognition and nuclear export

doi: 10.1038/s41698-024-00737-0

Figure Lengend Snippet: a – d CRC cells were exposed to specific siRNAs or plasmids. RNA level, protein level, cell viability and migration capability were detected by RT-qPCR ( a ), immunoblot ( b ), CCK-8 ( c ) and Transwell ( d ) assays, respectively. e HCT116 cells transduced with lentivirus vectors were subcutaneously inoculated into Balb/c nude mice ( n = 5). Tumors were stripped, weighed and measured three weeks after the implantations. Data were presented as the mean ± SD. ALY ALYREF. ** P < 0.01; *** P < 0.001; two-sided Student’s t test.

Article Snippet: Transwell® Permeable Supports (Corning #3422, NY) were employed to assess cell migration capability.

Techniques: Migration, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Transduction

a Transcriptome analysis was conducted in DLD-1 cells treated with siALYREF or siNC. KEGG pathway enrichment analysis revealed the top five significant ALYREF-activated pathways and oncogenes annotated in the mTOR pathway. b Venn plot showing the overlay of ALYREF-RIP-bis-seq data from Yang et al., ALYREF-upregulated genes and oncogenes annotated in mTOR signaling from our RNA-seq data. c , d Transcripts screened were reverified with RT-qPCR and immunoblot analysis. RPS6KB2 and RPTOR encode S6K2 and Raptor protein, respectively. e , f Cell proliferation and migration capabilities in rescue assays were assessed by CCK8 ( e ) and Transwell ( f ) techniques, respectively. Data were presented as the mean ± SD. ALY ALYREF. * P < 0.05; ** P < 0.01; *** P < 0.001; ns not significant; two-tailed Student’s t test.

Journal: NPJ Precision Oncology

Article Title: ALYREF recruits ELAVL1 to promote colorectal tumorigenesis via facilitating RNA m5C recognition and nuclear export

doi: 10.1038/s41698-024-00737-0

Figure Lengend Snippet: a Transcriptome analysis was conducted in DLD-1 cells treated with siALYREF or siNC. KEGG pathway enrichment analysis revealed the top five significant ALYREF-activated pathways and oncogenes annotated in the mTOR pathway. b Venn plot showing the overlay of ALYREF-RIP-bis-seq data from Yang et al., ALYREF-upregulated genes and oncogenes annotated in mTOR signaling from our RNA-seq data. c , d Transcripts screened were reverified with RT-qPCR and immunoblot analysis. RPS6KB2 and RPTOR encode S6K2 and Raptor protein, respectively. e , f Cell proliferation and migration capabilities in rescue assays were assessed by CCK8 ( e ) and Transwell ( f ) techniques, respectively. Data were presented as the mean ± SD. ALY ALYREF. * P < 0.05; ** P < 0.01; *** P < 0.001; ns not significant; two-tailed Student’s t test.

Article Snippet: Transwell® Permeable Supports (Corning #3422, NY) were employed to assess cell migration capability.

Techniques: RNA Sequencing, Quantitative RT-PCR, Western Blot, Migration, Two Tailed Test

$a control and treated CRC cells were harvested for RIP assays with anti-ALYREF or control anti-IgG antibodies. RIP enrichment of certain samples was quantified and normalized to the input. b , c pmirGLO reporter plasmids (RPS6KB2-wt/mut or RPTOR-wt/mut) were co-transfected with plasmids/siRNAs as indicated for dual luciferase reporter assays. The relative luciferase activity of each sample was calculated as the ratio of firefly luciferase activity to Renilla luciferase activity and normalized to siNC. d – g RNA nucleocytoplasmic shuttling, RNA expression level, growth ability and migration capability were quantified by subcellular fractionation ( d ), RT-qPCR ( e ), CCK-8 ( f ) and Transwell ( g ) techniques in rescue experiments, respectively. Data were presented as the mean ± SD. ALY ALYREF. Rescue, siALYREF + ELAVL1. ** P < 0.01; *** P < 0.001; two-sided Student’s t test.$

Journal: NPJ Precision Oncology

Article Title: ALYREF recruits ELAVL1 to promote colorectal tumorigenesis via facilitating RNA m5C recognition and nuclear export

doi: 10.1038/s41698-024-00737-0

Figure Lengend Snippet: a control and treated CRC cells were harvested for RIP assays with anti-ALYREF or control anti-IgG antibodies. RIP enrichment of certain samples was quantified and normalized to the input. b , c pmirGLO reporter plasmids (RPS6KB2-wt/mut or RPTOR-wt/mut) were co-transfected with plasmids/siRNAs as indicated for dual luciferase reporter assays. The relative luciferase activity of each sample was calculated as the ratio of firefly luciferase activity to Renilla luciferase activity and normalized to siNC. d – g RNA nucleocytoplasmic shuttling, RNA expression level, growth ability and migration capability were quantified by subcellular fractionation ( d ), RT-qPCR ( e ), CCK-8 ( f ) and Transwell ( g ) techniques in rescue experiments, respectively. Data were presented as the mean ± SD. ALY ALYREF. Rescue, siALYREF + ELAVL1. ** P < 0.01; *** P < 0.001; two-sided Student’s t test.

Article Snippet: Transwell® Permeable Supports (Corning #3422, NY) were employed to assess cell migration capability.

Techniques: Control, Transfection, Luciferase, Activity Assay, RNA Expression, Migration, Fractionation, Quantitative RT-PCR, CCK-8 Assay

a , b Candidate transcriptional factors bound to the ALYREF promoter were predicted in silico ( a ) and verified with RT-qPCR ( b ). c Schematic plot of the putative E2F6 binding site in the ALYREF promoter. d Naïve cells were harvested for ChIP assays with anti-E2F6 or anti-IgG antibodies. ChIP enrichment was quantified by RT-qPCR with specific primers for the ALYREF promoter and normalized to the input. e pGL3 reporter plasmid carrying the ALYREF-wt/mut promoter was co-transfected with Renilla reporter plasmid and siRNAs as indicated for dual luciferase reporter assays. Relative luciferase activity was calculated as the ratio of firefly luciferase activity to Renilla luciferase activity and normalized to siNC. f , g Growth and migration capabilities were quantified by CCK-8 ( f ) and Transwell ( g ) techniques in rescue experiments, respectively. Data were presented as the mean ± SD. ALY ALYREF. *** P < 0.001; ns not significant; two-sided Student’s t test.

Journal: NPJ Precision Oncology

Article Title: ALYREF recruits ELAVL1 to promote colorectal tumorigenesis via facilitating RNA m5C recognition and nuclear export

doi: 10.1038/s41698-024-00737-0

Figure Lengend Snippet: a , b Candidate transcriptional factors bound to the ALYREF promoter were predicted in silico ( a ) and verified with RT-qPCR ( b ). c Schematic plot of the putative E2F6 binding site in the ALYREF promoter. d Naïve cells were harvested for ChIP assays with anti-E2F6 or anti-IgG antibodies. ChIP enrichment was quantified by RT-qPCR with specific primers for the ALYREF promoter and normalized to the input. e pGL3 reporter plasmid carrying the ALYREF-wt/mut promoter was co-transfected with Renilla reporter plasmid and siRNAs as indicated for dual luciferase reporter assays. Relative luciferase activity was calculated as the ratio of firefly luciferase activity to Renilla luciferase activity and normalized to siNC. f , g Growth and migration capabilities were quantified by CCK-8 ( f ) and Transwell ( g ) techniques in rescue experiments, respectively. Data were presented as the mean ± SD. ALY ALYREF. *** P < 0.001; ns not significant; two-sided Student’s t test.

Article Snippet: Transwell® Permeable Supports (Corning #3422, NY) were employed to assess cell migration capability.

Techniques: In Silico, Quantitative RT-PCR, Binding Assay, Plasmid Preparation, Transfection, Luciferase, Activity Assay, Migration, CCK-8 Assay

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transwell permeable supports 3422 (Corning Life Sciences)

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1) Product Images from "Canonical microRNA loss drives tumor development implicating therapeutic efficacy of enoxacin in angiosarcoma"

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